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Azobenzene reductase
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Azobenzene reductase : ウィキペディア英語版
Azobenzene reductase

Azobenzene reductase also known as azoreductase () is an enzyme that catalyzes the chemical reaction:
:N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ \rightleftharpoons 4-(dimethylamino)azobenzene + NADPH + H+
The 3 substrates of this enzyme are N,N-dimethyl-1,4-phenylenediamine, aniline, and nicotinamide adenine dinucleotide phosphate ion, whereas its 3 products are 4-(dimethylamino)azobenzene, nicotinamide adenine dinucleotide phosphate, and hydrogen ion.
This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor.
== Mechanism ==

The reaction catalyzed by this enzyme proceeds via a ping-pong mechanism by using 2 equivalents of NAD(P)H to reduce one equivalent of the azo compound substrate (for example methyl red where Ar = ''p''-dimethylaniline and Ar' = ''o''-benzoic acid) into two equivalents of aniline product:
: Ar–N=N–Ar' + 2(NAD(P)H + H+) \rightleftharpoons Ar–NH2 + NH2–Ar' + 2NAD(P)+

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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